LOCUS       sequence-90231-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human codon optimized Csy4 and FokI-dCas9 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pSQT1601
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(61..126)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(133..153)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtgc ggccgcttca
       61 cttgtcatcg tcatccttgt aatcgatgtc atgatcttta taatcaccgt catggtcttt
      121 gtagtcgctc gagactttcc tcttcttctt gggggatccg tcacccccaa gctgtgacaa
      181 atctatccga gtttcatata atcccgtgat ggattggtga atcagtgtcg cgtctagcac
      241 ctccttggta gaagtgtatc gtttgcgatc tatcgttgtg tcaaaatact tgaatgcggc
      301 tggagcgccg aggttggtaa gagtaaacaa atggataata ttttccgcct gctcacgtat
      361 gggtttatcc ctgtgcttgt tgtatgcgct taatactttg tccagattgg catcagctag
      421 gatgactctc ttactgaatt ccgaaatttg ctctatgatt tcgtcgagat aatgtttgtg
      481 ctgctcaaca aaaagttgct tctgttcgtt atcttcaggt gaacctttca acttctcgta
      541 atgggacgct aaatacagga aattcacgta tttagacggt agtgcgagtt cgttcccctt
      601 ttgaagctct ccgg
//
