LOCUS       sequence-90235-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Csy4 and Cas9 nuclease expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin
            MJ, Aryee MJ, Joung JK
  TITLE     Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome 
            editing.
  JOURNAL   Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908.
  PUBMED    24770325
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908."
COMMENT     Sequence Label: pSQT834
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..600
                     /codon_start=1
                     /product="CRISPR-associated endoribonuclease responsible 
                     for pre-crRNA processing in Pseudomonas aeruginosa 
                     (Haurwitz et al., 2010)"
                     /label=Csy4
                     /translation="MDHYLDIRLRPDPEFPPAQLMSVLFGKLHQALVAQGGDRIGVSFP
                     DLDESRSRLGERLRIHASADDLRALLARPWLEGLRDHLQFGEPAVVPHPTPYRQVSRVQ
                     AKSNPERLRRRLMRRHDLSEEEARKRIPDTVARALDLPFVTLRSQSTGQHFRLFIRHGP
                     LQVTAEEGGFTCYGLSKGGFVPWF"
     CDS             601..654
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggaccacta cctcgacatt
       61 cgcttgcgac cggacccgga atttcccccg gcgcaactca tgagcgtgct cttcggcaag
      121 ctccaccagg ccctggtggc acagggcggg gacaggatcg gcgtgagctt ccccgacctc
      181 gacgaaagcc gctcccggct gggcgagcgc ctgcgcattc atgcctcggc ggacgacctt
      241 cgtgccctgc tcgcccggcc ctggctggaa gggttgcggg accatctgca attcggagaa
      301 ccggcagtcg tgcctcaccc cacaccgtac cgtcaggtca gtcgggttca ggcgaaaagc
      361 aatccggaac gcctgcggcg gcggctcatg cgccggcacg atctgagtga ggaggaggct
      421 cggaaacgca ttcccgatac ggtcgcgaga gccttggacc tgcccttcgt cacgctacgc
      481 agccagagca ccggacagca cttccgtctc ttcatccgcc acgggccgtt gcaggtgacg
      541 gcagaggaag gaggattcac ctgttacggg ttgagcaaag gaggtttcgt tccctggttc
      601 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaatggat
      661 aaaaagtatt ctattggttt agacatcggc actaattccg ttggatgggc tgtcataacc
      721 gatgaataca aagtaccttc aaagaaa
//
