LOCUS       sequence-90237-e        1165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR based C-terminal SRM tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1165)
  TITLE     Sakata Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-SRM (sortase recognition motif)-hphMX4
FEATURES             Location/Qualifiers
     source          1..1165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(94..291)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 cgcggccgca taggccacta gtggatctga tatcatcgat gaattcgagc tcgttttcga
       61 cactggatgg cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg
      121 attgacgcat gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag
      181 gcgaaaaaaa atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa
      241 aaactataca aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattat
      301 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
      361 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
      421 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
      481 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
      541 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
      601 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
      661 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
      721 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
      781 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
      841 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
      901 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
      961 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     1021 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     1081 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     1141 cgatctttgt agaaaccatc ggcgc
//
