LOCUS       sequence-90238-e        1146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PCR based C-terminal SRM tagging in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1146)
  TITLE     Sakata Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-SRM (sortase recognition motif)-kanMX6
FEATURES             Location/Qualifiers
     source          1..1146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      101..288
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(156..180)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        363..706
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(774..793)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aagcttcgta cgctgcaggt cgacggatcc ccgggttaat taccgggtct ctaccagaaa
       61 ctggcggcgg tagacgttag ggcgcgccac ttctaaataa gcgaatttct tatgatttat
      121 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc
      181 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct
      241 ttctcaggta tagtatgagg tcgctcttat tgaccacacc tctaccggca gatccgctag
      301 ggataacagg gtaatataga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
      361 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
      421 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
      481 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
      541 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
      601 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
      661 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa
      721 gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
      781 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
      841 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
      901 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
      961 tatccgtact cctgatgatg catggttact caccactgcg atccccggca aaacagcatt
     1021 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     1081 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     1141 tcgtct
//
