LOCUS       sequence-9024-ex        4504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pDR3.01
FEATURES             Location/Qualifiers
     source          1..4504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(73..100)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(192..278)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    331..430
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2680..2779)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2891..2910)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2902..3711
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2969..2988)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3854..4442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4343..4362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt
       61 tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca
      121 gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc
      181 gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
      241 aaggcccagt cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct
      301 cgcgttaacg ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg
      361 acctgttcgt tgcaacaaat tgatgagcaa tgctttttta taatgccaag tttgtacaaa
      421 aaagcaggct ccatgcaaaa cacacagata tttactaact tcgctcacag agcacatgat
      481 gggtatgttt atcattttta aatgttggta ttaagtattt ctttttcaga ttaccgttta
      541 acagtgcgaa tccttccaac aaagatccaa ttttcacaat gccaatatcg gtcaaaccga
      601 aaactcgtga gccggcttct ttcacagata acaggtaaat ttgtcgtgcc gttttaatga
      661 attcatcaaa aaacttttag gatatatgtc agcaatattc ccttctcgtt tcgtgaacaa
      721 gatttggcgg caatgttctt cgcgtgagtt ttcaatttac aacaagttct gaataagaat
      781 attaatttca gatatggaag agtcctgagt gtggaaatcg tcacaaatga tcgtggatcc
      841 aaagggttcg ggtttgtcac actcgattcc atcgaatcct gtgagaaagc tcgtgctgcg
      901 cttcacgaat cacatgttca aggaagaatt atagaagtga gaagagcgac accaacccgc
      961 agaaagctta tcaacaatcc acaaaatgaa gttttgccac caccaaagct gtgtgtcgat
     1021 cttcgagccc ctcataattt atggagagct gagccaatgc atcagttgtt caaggaaaag
     1081 gagaacacaa catgttttcc cgaagctgga ttcatgatgg caccataccg tagcaatgga
     1141 attttcaaca cgcgtagtct tgtgcagacc aaaccacctc gatgcaccaa gcacagcgag
     1201 ctcaagcttt cttcagctgg tgaatacttc tgcaaaaacg gcgagcctac gacggaaaca
     1261 agtattctga tgtgcatgca cagacaaaac tcaccatgca gcaataagtg ttctgattct
     1321 tcgaatcacg agctgtctga tgtggagttg aactctatat tcccacatca tcttcgtgac
     1381 cagattactg ctcttctcga cacttcaaac cattttggat caggaaataa tagtgctaac
     1441 aaaggaaaga gagcaccatc tgtgacatct tctggattga gatcatcaga gagcgagaca
     1501 gtttcaggta aatagtttag attctagatt gtcgattttc atttaaaaat caaattttag
     1561 acgaagagat tcattggtcc ccacataaca gccctgatta tcttctcgct gctctctacg
     1621 aaggttccac atcgttccac ggaaagtctg tttctccacc aaaagaatcg tcaagccagt
     1681 aatcagttca actgatacgc ccattttatt tctactaact gcgtgttttg cattttatcg
     1741 aacaccccta aactttcact taaccaaaaa gctttaacaa attttttacg gaatcttctt
     1801 gtacagcgac actcgttgtc cttgtcaaat gattcgttgc tagtttttct tccctgtagc
     1861 ttctatatcg acccccccgg tgttattttg taggaatgcg tttcagcatc ttccctattt
     1921 tgccatctct cacatacact caacattagc aatcggaaaa cagatcttct atattgaaaa
     1981 tatactacca gtccgtttaa cctacctcgc ttgctaacat tattgaatgt gcccacgtat
     2041 tgtattttct gagcatcttt ctgttttttt tgtattttat agacgctgtg ttcgccagtt
     2101 atttttgcat tcctgtattg ctcgaagtca aatgaagtgc ttcgccatat tcaattgttt
     2161 tattttcaaa atcacccgtt aatactagat ttcagtttga atttaatcac agttgtgaaa
     2221 gatcattatc ccggcgtgtc attgagagat caaatgttat ctttcggcac ctcttggcct
     2281 tcctccattc atgatcagaa gattcactct cttctaaaat gacgtcgtga gagactgcgg
     2341 gaagagaaaa ggacgatggc tgtcggcact ttgtggatgc tgctggaaaa gtttggtgaa
     2401 ttttgaagtt ttgtggtttt tgatgataac ttcaaaatgg atgggaaaac acgagagaga
     2461 gagagaaact tacggatttg aagagtgggt ggagaggaag ggtacaaact gcttcgctcc
     2521 tcatcatttc ctgtgccacg tcgacttcga ttttgacgac ttgaaagtgt tgcgaagaag
     2581 gacattcgac gggatttcga tcgtcgacaa ccaccgctta cccaacaaaa taggcacgaa
     2641 acaggaatga agatacctga aataaatatg cattattaga cccagctttc ttgtacaaag
     2701 tgggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2761 aaaataaaat cattatttgc catccagctg cagctctggc ccgtgtctca aaatctctga
     2821 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     2881 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta
     2941 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     3001 tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa
     3061 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     3121 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     3181 ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat
     3241 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     3301 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     3361 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     3421 gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact
     3481 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     3541 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     3601 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     3661 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg
     3721 gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca
     3781 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3841 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3901 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3961 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4021 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4081 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4141 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4201 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4261 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4321 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4381 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4441 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4501 tgtt
//
