LOCUS       sequence-90240-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
  TITLE     PUMA induces the rapid apoptosis of colorectal cancer cells.
  JOURNAL   Mol Cell. 2001 Mar . 7(3):673-82.
  PUBMED    11463391
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2001 Mar . 7(3):673-82."
COMMENT     Sequence Label: pHA-PUMA
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(653..679)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     primer_bind     complement(730..754)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(751..802)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(780..800)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ttggatctta taggaggtgg gaggggcctg ccccccgagt ccctgacgtc caccgggcgg
       61 gtgcatgcac ctaattgggc tccatctcgg gggctctgtg gcccctgggt aagggcagga
      121 gtcccatgat gagattgtac aggaccctcc agggtgaggg gcggtgccgc tgctgctcct
      181 cttgtctccg ccgctcgtac tgtgcgttga ggtcgtccgc catccgccgc agctgggccc
      241 cgatctcccg ggcccactgt tcctcctccc cgcggactcc cggggccgcc tgggtgggac
      301 cgcccgccag agcccccggg gcgctgggca cgggcgactc caggtgctgc tccgccagcg
      361 agagcgaggg ctgaggaccg tccgggcgcg ggcctcgggg ccggctgcgg ggacccccag
      421 gccagcggga accccccagg gcggcggtga cggcgggtgg ggcggtgggg gcgcagaggt
      481 aggcagcggg cagcagggtg ggggcggcgg gggcggcagc caggccgggc tcgcagaggc
      541 cgcaggacac tgccgagggc accaggcggc cgagcgggaa ggggcgcggg ccgtcgcggg
      601 ccaggccctc tacgggctcc ggggagctgc cctcctggcg tgcgcgggcc atcaagcttg
      661 cgtaatctgg aacatcgtac gaagcgtaat ctggaacatc gtatcccatt tcggtaccca
      721 gcttctagag atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac
      781 acgcctaccg cccatttgcg tcaacggggc ggggttatta cgacattttg gaaaagtccc
      841 gt
//
