LOCUS       sequence-90241-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
  TITLE     PUMA induces the rapid apoptosis of colorectal cancer cells.
  JOURNAL   Mol Cell. 2001 Mar . 7(3):673-82.
  PUBMED    11463391
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2001 Mar . 7(3):673-82."
COMMENT     Sequence Label: pHA-PUMA
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..105
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    753..888
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(777..796)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     832..851
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagcagagct gttagtgacc gtcagatctc tagaagctgg gtaccgaaat gggatacgat
       61 gttccagatt acgcttcgta cgatgttcca gattacgcaa gcttgatggc ccgcgcacgc
      121 caggagggca gctccccgga gcccgtagag ggcctggccc gcgacggccc gcgccccttc
      181 ccgctcggcc gcctggtgcc ctcggcagtg tcctgcggcc tctgcgagcc cggcctggct
      241 gccgcccccg ccgcccccac cctgctgccc gctgcctacc tctgcgcccc caccgcccca
      301 cccgccgtca ccgccgccct ggggggttcc cgctggcctg ggggtccccg cagccggccc
      361 cgaggcccgc gcccggacgg tcctcagccc tcgctctcgc tggcggagca gcacctggag
      421 tcgcccgtgc ccagcgcccc gggggctctg gcgggcggtc ccacccaggc ggccccggga
      481 gtccgcgggg aggaggaaca gtgggcccgg gagatcgggg cccagctgcg gcggatggcg
      541 gacgacctca acgcacagta cgagcggcgg agacaagagg agcagcagcg gcaccgcccc
      601 tcaccctgga gggtcctgta caatctcatc atgggactcc tgcccttacc caggggccac
      661 agagcccccg agatggagcc caattaggtg cctgcacccg cccggtggac gtcagggact
      721 cggggggcag gcccctccca cctcctgata aggatccaga catgataaga tacattgatg
      781 agtttggaca aaccacaact agaatgcagt gaaaaaaaat gctttatttg tgaaatttgt
      841 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaaacaa
      901 ttgcattcat tttatgt
//
