LOCUS       sequence-90242-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Yu J, Zhang L, Hwang PM, Kinzler KW, Vogelstein B
  TITLE     PUMA induces the rapid apoptosis of colorectal cancer cells.
  JOURNAL   Mol Cell. 2001 Mar . 7(3):673-82.
  PUBMED    11463391
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2001 Mar . 7(3):673-82."
COMMENT     Sequence Label: pHA-PUMA
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..49
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    697..831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(721..740)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     775..794
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tagatgttca gattacgctt cgtacgatgt tccagattac gcaagcttga tggcccgcgc
       61 acgccaggag ggcagctccc cggagcccgt agagggcctg gcccgcgacg gcccgcgccc
      121 cttcccgctc ggccgcctgg tgccctcggc agtgtcctgc ggcctctgcg agcccggcct
      181 ggctgccgcc cccgccgccc ccaccctgct gcccgctgcc tacctctgcg cccccaccgc
      241 cccacccgcc gtcaccgccg ccctgggggg ttcccgctgg cctgggggtc cccgcagccg
      301 gccccgaggc ccgcgcccgg acggtcctca gccctcgctc tcgctggcgg agcagcacct
      361 ggagtcgccc gtgcccagcg ccccgggggc tctggcgggc ggtcccaccc aggcggcccc
      421 gggagtccgc ggggaggagg aacagtgggc ccgggagatc ggggcccagc tgcggcggat
      481 ggcggacgac ctcaacgcac agtacgagcg gcggagacaa gaggagcagc agcggcaccg
      541 cccctcaccc tggagggtcc tgtacaatct catcatggga ctcctgccct tacccagggg
      601 ccacagagcc cccgagatgg agcccaatta ggtgcctgca cccgcccggt ggacgtcagg
      661 gactcggggg gcaggcccct cccacctcct gataaggatc cagacatgat aagatacatt
      721 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
      781 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
      841 aattgcattc atttttatgt ttcaggtt
//
