LOCUS       sequence-90244-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of 2xFlag-tagged human Large tumor suppressor kinase 1 
            (LATS1) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Oka T, Mazack V, Sudol M
  TITLE     Mst2 and Lats kinases regulate apoptotic function of Yes 
            kinase-associated protein (YAP).
  JOURNAL   J Biol Chem. 2008 Oct 10;283(41):27534-27546. doi: 
            10.1074/jbc.M804380200. Epub 2008 Jul 17.
  PUBMED    18640976
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M804380200"; journalName: "J Biol Chem"; date: 
            "2008-10-10- 10"; volume: "283"; issue: "41"; pages: "27534-27546"
COMMENT     Sequence Label: p2xFlag CMV2 Lats1
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..63
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcgagctcgt ttgtgaaccg tcagaattga tctaccatgg actacaaaga cgatgacgac
       61 aagcttgcgg ccgcgaattc aagcttagcc accatggact acaaagacga tgacgataaa
      121 gcaaggctcg aatcggtacc aatgaagagg agtgaaaagc cagaaggata tagacaaatg
      181 aggcctaaga cctttcctgc cagtaactat actgtcagta gccggcaaat gttacaagaa
      241 attcgggaat cccttaggaa tttatctaaa ccatctgatg ctgctaaggc tgagcataac
      301 atgagtaaaa tgtcaaccga agatcctcga caagtcagaa atccacccaa atttgggacg
      361 catcataaag ccttgcagga aattcgaaac tctctgcttc catttgcaaa tgaaacaaat
      421 tcttctcgga gtacttcaga agttaatcca caaatgcttc aagacttgca agctgctgga
      481 tttgatgagg atatggttat acaagctctt cagaaaacta acaacagaag tatagaagca
      541 gcaattgaat tcattagtaa aatgagttac caagatcctc gacgagagca gatggctgca
      601 gcagctgcca gacctattaa tgccagcatg aaaccaggga atgtgcagca atcagttaac
      661 cgcaaacaga gctggaaagg ttctaaagaa tccttagttc ctcagaggca tggcccgcca
      721 ctaggagaaa gtgtggccta tcattctgag agtcccaact cacagacaga tgtaggaaga
      781 cctttgtctg gatctggtat atcagcattt gttcaagctc accctagcaa cggacagaga
      841 gtgaaccccc caccaccacc tcaggtaagg agtgttactc ctccaccacc tccaa
//
