LOCUS       sequence-90246-e        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TEV SNAPf Prescission TwinStrepII clo...
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             683..706
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             716..739
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             776..799
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(1087..1106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgttttcgt aacagttttg taataaaaaa acctataact attccggatt attcataccg
       61 tcccaccatc gggcgcggat ccctttaaga aggagatata gttaacctct acttccaatc
      121 cggctctgat aaagactgcg agatgaagcg cacgacgctc gattcgcccc ttggcaaatt
      181 ggaactgagc ggttgtgagc aaggtctgca tcgtatcatc tttctgggaa agggaactag
      241 tgcagcagat gcagtcgagg tcccggctcc tgctgccgtg cttggtggtc ccgagccttt
      301 gatgcaagct accgcttggc tgaatgcata tttccatcag ccagaggcga tcgaggagtt
      361 ccccgttccc gcgctccatc atccggtttt ccaacaggaa tccttcaccc gccaggtcct
      421 gtggaagctc ctgaaggtcg taaagtttgg tgaagtgatc tcatacagtc acttggccgc
      481 cctggcgggc aacccagccg ctactgctgc cgttaagacc gcactcagtg gcaaccctgt
      541 acctatcctg atcccttgtc atagagtcgt ccaaggcgac ctggacgtgg gaggttacga
      601 gggcggtctg gctgtgaaag aatggctgtt ggcacatgag ggccatcgtc tgggcaaacc
      661 tggccttgga cccgccggtg gtttggaggt actgttccag ggcccgggag cttcatggtc
      721 tcacccccaa tttgagaaag gcggtggcag cggcggcggt agcggaggtg gcagctggtc
      781 acacccacaa ttcgagaaat aacggtccga agcgcgcgga attcaaaggc ctacgtcgac
      841 gagctcacta gtcgcggcct catgatctcc taggggccgc tttcgaatct agagcctgca
      901 gtctcgaggc atgcggtacc aagcttgtcg agaagtacta gaggatcata atcagccata
      961 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1021 aacataaaat gaatgcaatt gttgttgttt acttgtttat tgcagcttat aatggttaca
     1081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt ttt
//
