LOCUS       sequence-90250-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Gradia SD, Ishida JP, Tsai MS, Jeans C, Tainer JA, Fuss JO
  TITLE     MacroBac: New Technologies for Robust and Efficient Large-Scale 
            Production of Recombinant Multiprotein Complexes.
  JOURNAL   Methods Enzymol. 2017;592:1-26. doi: 10.1016/bs.mie.2017.03.008. 
            Epub 2017 May 15.
  PUBMED    28668116
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2017"; volume: "592:1-26. doi"; pages: " 
            10.1016/bs.mie.2017.03.008. Epub 2017 May 15"
COMMENT     Sequence Label: pFastBac TEV SNAPf Prescission TwinStrepII clo...
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     mobile_element  261..426
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(524..543)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     578..597
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(885..908)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(945..968)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(978..1001)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
       61 gccgctacag ggcgcgtccc attcgccatt caggctgcaa ataagcgttg atattcagtc
      121 aattacaaac attaataacg aagagatgac agaaaaattt tcattctgtg acagagaaaa
      181 agtagccgaa gatgacggtt tgtcacatgg agttggcagg atgtttgatt aaaaacataa
      241 caggaagaaa aatgccccgc tgtgggcgga caaaatagtt gggaactggg aggggtggaa
      301 atggagtttt taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc
      361 gtcgtaagct aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat
      421 ctggttcgga tctcccctgc aggcgtttaa acctaactcc atttaaatgg tggatgtttc
      481 taggctcaag cagtgatcag atccagacat gataagatac attgatgagt ttggacaaac
      541 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      601 atttgtaacc attataagct gcaataaaca agtaaacaac aacaattgca ttcattttat
      661 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      721 tggtatggct gattatgatc ctctagtact tctcgacaag cttggtaccg catgcctcga
      781 gactgcaggc tctagattcg aaagcggccc ctaggagatc atgaggccgc gactagtgag
      841 ctcgtcgacg taggcctttg aattccgcgc gcttcggacc gttatttctc gaattgtggg
      901 tgtgaccagc tgccacctcc gctaccgccg ccgctgccac cgcctttctc aaattggggg
      961 tgagaccatg aagctcccgg gccctggaac agtacctcca aaccaccggc gggtccaagg
     1021 ccaggtttgc ccagacgatg gccctcatgt gccaacagcc attctttcac agccagaccg
     1081 ccctcgtaac ctcccacgt
//
