LOCUS       sequence-90284-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for disruption of genes by CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pSAG1::CAS9-U6::sgUPRT
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..36
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_RNA        complement(61..135)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     717..733
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     717..733
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctgga gctcaaaaaa
       61 gcaccgactc ggtgccactt ttcaagttga taacggacta gccttatttt aacttgctat
      121 ttctagctct aaaacgctct cacaatcgag acgccaactt gacatcccca tttaccagaa
      181 ggcaaacacc cccttcgggg acgaggtgac cctgcgcgac agaaagcccc ttcgaagagc
      241 gcacagggag gaagcaggcc tctgcaggtc gccatttgaa aatctgacag aactgatgga
      301 aatatgattc ttgtcagaga agacattcga gagttcgaag gtttccccct tggctctaca
      361 tatcccagtg tctcgcgttc tgcaggaggc gcgtcaggcc taggatgcaa tattggcgcc
      421 caattcacag tgcagcggcg cagccgtcgc aacacttcgc agcgtgaaag acttatcgct
      481 ctccgcctct cgatggttcg ggatgttcgc ttcgagtgta tgctgcgcaa gctttcgcgc
      541 ttgggtacgt taatttgaaa tctttcgcgt ttcccgtgtg tcctcagtgt ctccaacatg
      601 tggccgcggt gcaatcgtcc ccacgacgat ttcaactcgc gcactttgtc tcgtcgcacc
      661 ctcccggaaa tacagcgtgc ttctgcttac ttgagctcca ccgcggtggc ggccgctcta
      721 gaactagtgg atccccctcg ggggggcaag aattgtgtta accggttcga ctaaaacaac
      781 tgatagctgt cttgtgtatt gacccatgtg gcgcgtgtgt cgacggcgat caacatcagc
      841 caggtccgcc acacatccgt tcagagattc ggacccagtc ataaactttc gctgcttgct
      901 gcgaaacact cctatttaga gtcggccggt ttttgtggat gtgacacctg caagccacaa
      961 cggaacaact cagtttcttt ataatggggc acatgctgca cgaagtgtgt ttcctttgtc
     1021 gatttgagaa gtgagcacaa cggtgattaa ttaactactt gtacagctcg tccatgccga
     1081 gagtgatccc ggcggcggtc
//
