LOCUS       sequence-90285-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for disruption of genes by CRISPR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Shen B, Brown KM, Lee TD, Sibley LD
  TITLE     Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii 
            Using CRISPR/CAS9.
  JOURNAL   MBio. 2014 May 13;5(3). pii: e01114-14. doi: 10.1128/mBio.01114-14.
  PUBMED    24825012
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-05-13"; volume: "5(3). pii: e01114-14. doi"; pages: " 
            10.1128/mBio.01114-14"
COMMENT     Sequence Label: pSAG1::CAS9-U6::sgUPRT
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..53
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     38..54
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc
       61 ttttacatcc gttgcctttt ccacggtccg tgatttcatg tgcgtgcagc ttcaaagact
      121 ggtcgttgcg actaataaga ctgcagtgac aggtcgaatg gtgggcacct tgctgatgac
      181 tatctactgc aaagtctgag acaacgaacg aaacttccca cacgaggcat ttgaaactga
      241 cggtgtctag gtaatatgca ctgcaagaca cggtactggg gcctcgctga attaggggcc
      301 gatctcgttg ccctatcagt gctcacagtg ccgcaacgta acaccagggc aggttcttga
      361 cagtggcaac aatgtgcgac gggcgtgtga acgtttcgta gtcatagcgc tagcacgtac
      421 ctagccacat ggtcgtgagg agctttacca tgcgtctaga aggtggatgc gggacacgcc
      481 ttcctggcct ttggctcccg agacgcgtgt tctaaccaca aaccttgaga cgcgtgttcc
      541 aaccacgcac cctgacacgc gtgttccaac cacgcaccct gagacgcgtg ttctaaccac
      601 gcaccctgag acgcgtgttc taaccacgca ccctgagacg cgtgttctgc cgcacaatgt
      661 gcacctgtag gaagctgtag tcactgctga ttctcactgt tctcggcaag ggccgacgac
      721 cggagtacag tttttgtggg cagagccgtt gtgcagcttt ccgttcttct cggttgtgtc
      781 acatgtgtca ttgtcgtgta aacacacggt tgtatgtcgg tttcgctgca ccacttcatt
      841 atttcttctg gttttttgac gagtatgcat ctagacaaaa tggacaagaa gtacagcatc
      901 ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg
      961 cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatca
//
