LOCUS       sequence-90291-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP reporter for cytoplasmic inclusions formed by the fused, 
            C-terminal region of protein muNS from mammalian orthoreovirus Type 
            3 Dearing .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Broering TJ, Arnold MM, Miller CL, Hurt JA, Joyce PL, Nibert ML
  TITLE     Carboxyl-proximal regions of reovirus nonstructural protein muNS 
            necessary and sufficient for forming factory-like inclusions.
  JOURNAL   J Virol. 2005 May;79(10):6194-206.
  PUBMED    15858004
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2005-05"; volume: "79(10)"; pages: "6194-206"
COMMENT     Sequence Label: pEGFP-C1-M3(T3D)471-721
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(20..448)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(130..151)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     342..361
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        475..579
                     /gene="bla"
                     /label=AmpR promoter
     promoter        581..938
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        581..772
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(604..624)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      789..924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
       61 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
      121 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
      181 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
      241 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
      301 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
      361 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
      421 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
      481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      541 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
      601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
      661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
      721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
      781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
      841 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
      901 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
      961 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     1021 ga
//
