LOCUS       sequence-90295-e        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human SUMO3 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET
  TITLE     Covalent modification of PML by the sentrin family of ubiquitin-like
            proteins.
  JOURNAL   J Biol Chem. 1998 Feb 6. 273(6):3117-20.
  PUBMED    9452416
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Feb 6. 273(6):3117-20."
COMMENT     Sequence Label: pcDNA3/HA-SUMO3 (Sentrin 2)
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             116..910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(170..189)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     780..799
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
       61 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
      121 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      181 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      241 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
      301 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      361 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      421 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      481 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      541 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      601 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
      661 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      721 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      781 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
      841 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
      901 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
      961 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1021 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1081 cccaacttgt ttattgcagc ttataatggt tac
//
