LOCUS       sequence-90300-e        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human SUMO3 with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Kamitani T, Nguyen HP, Kito K, Fukuda-Kamitani T, Yeh ET
  TITLE     Covalent modification of PML by the sentrin family of ubiquitin-like
            proteins.
  JOURNAL   J Biol Chem. 1998 Feb 6. 273(6):3117-20.
  PUBMED    9452416
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1998 Feb 6. 273(6):3117-20."
COMMENT     Sequence Label: pcDNA3/HA-SUMO3 (Sentrin 2)
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             132..407
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKA
                     YCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
     promoter        complement(502..520)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(503..520)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(540..557)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    546..770
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(903..922)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1113..1134
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttatgtaccc atacgatgtt ccggattacg
      121 ctagcggatc catgtccgag gagaagccca aggagggtgt gaagacagag aatgaccaca
      181 tcaacctgaa ggtggccggg caggacggct ccgtggtgca gttcaagatc aagaggcaca
      241 cgccgctgag caagctgatg aaggcctact gcgagaggca gggcttgtca atgaggcaga
      301 tcagattcag gttcgacggg cagccaatca atgaaactga cactccagca cagctggaga
      361 tggaggacga ggacaccatc gacgtgttcc agcagcagac gggaggtgtg ccggagagca
      421 gcctggcagg gcacagtttc tgaattctgc agatatccat cacactggcg gccgctcgag
      481 catgcatcta gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc
      541 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
      601 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
      661 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
      721 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
      781 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
      841 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
      901 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
      961 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1021 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1081 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1141 actcaaccct atctcggtct attcttttga t
//
