LOCUS       sequence-90313-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid vector carrying N-Bio-1xproteinA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pCAndy-NBioTAP
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     191..213
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     205..222
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     206..222
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     232..251
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        232..250
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     275..291
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     276..292
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(620..793)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      121 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      181 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
      241 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
      301 ttaattaaaa gatctggata tcggcgcgcc gaattcgaac gccgatcttg attagacctt
      361 gaccaccttg aacagcatct ctttccttaa ctaacacctt ttcaactttt ccgtcagtag
      421 gagcgttaat ttctgtctcc attttcatag cctccagcac cagaactgtt tgaccagctt
      481 taacagtgtc accttctttt accagtatct tagaaacggt accagcaaga ggggcaggga
      541 tttcaccttc accggccttt ccagccgagg agccgctctc gagagaagca gttgtaggta
      601 tatcacaatt cgcgtctact ttcggcgcct gagcatcatt tagcttttta gcttctgcta
      661 aaaggttagc gctttggctt gggtcatctt ttaaactttg gatgaaggcg tttcgttgtt
      721 cttcgtttaa gttaggtaaa tgtaagatct catagaacgc gttttgttgt tctttgttga
      781 atttgttgtc cacggcttca tcgtgttgcg caaggcctgc catgggcggc cgccaccgcg
      841 gtggagctcc agctt
//
