LOCUS       sequence-90318-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral destination vector for C-Bio-1xproteinA constructs using 
            Gateway recombination System. Design for cDNA clones from the 
            CCSBHuman Orfeome Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pHAGE- TRE-DEST-CBioTAP
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..41)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(61..234)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     protein_bind    509..633
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 attaatcatt actaagcgta gtctgggacg tcgtatgggt attcgaaccg cctattatac
       61 tttcggcgcc tgagcaccat ttagcttttt agcttctgct aaaaggttag cgctttggct
      121 tgggtcatct tttaaacttt ggatgaaggc gtttcgttgt tcttcgttta agttaggtaa
      181 atgtaagatc tcatagaacg cgttttgttg ttctttgttg aatttgttgt ccacctcgag
      241 tgacgatcca acgccgatct tgattagacc ttgaccacct tgaacagcat ctctttcctt
      301 aactaacacc ttttcaactt ttccgtcagt aggagcgtta atttctgtct ccattttcat
      361 agcctccagc accagaactg tttgaccagc tttaacagtg tcaccttctt ttaccagtat
      421 cttagaaacg gtaccagcaa gaggggcagg gatttcacct tcaccggcct ttccagctgg
      481 gccctcttgg cttcctagct ttgcgcaaac cactttgtac aagaaagctg aacgagaaac
      541 gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac agactacata
      601 atactgtaaa acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa
      661 gggagcctga catttatatt ccccagaaca tcaggttaat ggcgttt
//
