LOCUS       sequence-90320-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral destination vector for N -Bio-1xproteinA constructs using
            Gateway recombination System. Design for cDNA clones from the 
            CCSBHuman Orfeome Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pHAGE- TRE-DEST-NBioTAP
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..33)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    74..198
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(239..544)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(341..360)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttactaagcg tagtctggga cgtcgtatgg gtattcgaac cgcgggccct ctaggctacc
       61 tagctatgcg caaaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa
      121 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
      181 acatatccag tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt
      241 atattcccca gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat
      301 cagccacttc ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc
      361 gccagctttc atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca
      421 gcagacgtgc actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac
      481 tctgtacatc cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact
      541 gcatttcacc agcccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg
      601 acctcagcca tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct
      661 tcattctgca tggttgtgct taccagaccg gagatattga catcatatat gccttgagca
      721 actgatagct gtcgctgtca actgtcactg taatacgctg ct
//
