LOCUS       sequence-90323-e         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral destination vector for C-Bio-1xproteinA constructs using 
            Gateway recombination System. Design for cDNA clones from the 
            CCSBHuman Orfeome Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pHAGE- TRE-DEST-CBioTAP
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..35
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     45..69
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    177..301
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(326..331,332..349,350..356)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(476..495)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac
       61 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
      121 cgatccagcc tccggactct agaggatccc taccggtgat atcctcgagc ccatcaacaa
      181 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt
      241 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat
      301 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg
      361 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa
      421 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc
      481 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt
      541 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc
      601 c
//
