LOCUS       sequence-90325-e         279 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral destination vector for N -Bio-1xproteinA constructs using
            Gateway recombination System. Design for cDNA clones from the 
            CCSBHuman Orfeome Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Alekseyenko AA, Gorchakov AA, Kharchenko PV, Kuroda MI
  TITLE     Reciprocal interactions of human C10orf12 and C17orf96 with PRC2 
            revealed by BioTAP-XL cross-linking and affinity purification.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Feb 18;111(7):2488-93. doi: 
            10.1073/pnas.1400648111. Epub 2014 Feb 3.
  PUBMED    24550272
REFERENCE   2  (bases 1 to 279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1400648111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-02-18- 18"; volume: "111"; issue: "7"; pages: "2488-93"
COMMENT     Sequence Label: pHAGE- TRE-DEST-NBioTAP
FEATURES             Location/Qualifiers
     source          1..279
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..30
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     40..64
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      168..177
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag tgaaccgtca
       61 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
      121 cagcctccgg actctagagg atccctaccg gtgatatcct cgaggccgcc accatggcag
      181 gccttgcgca acacgatgaa gccgtggaca acaaattcaa caaagaacaa caaaacgcgt
      241 tctatgagat cttacattta cctaacttaa acgaagaac
//
