LOCUS       sequence-90342-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of a 
            photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli 
            in response to the amber codon, TAG. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Chin JW, Martin AB, King DS, Wang L, Schultz PG
  TITLE     Addition of a photocrosslinking amino acid to the genetic code of 
            Escherichiacoli.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11020-4. Epub 2002 Aug 
            1.
  PUBMED    12154230
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-08-20"; volume: "99(17)"; pages: 
            "11020-4. Epub 2002 Aug 1"
COMMENT     Sequence Label: pEVOL-pBpF
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     156..173
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(229..246)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
       61 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
      121 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
      181 tcttctctca tccgccaaaa cagccaagct ggagaccgtt taaactcaat gatgatgatg
      241 atgatggtcg acttattata atctctttct aattggctct aaaatcttta taagttcttc
      301 agctacagca ttttttaaat ccattggatg caattcctta tttttaaata aactctctaa
      361 ctcctcatag ctattaactg tcaaatctcc accaaatttt tctggccttt ttatggttaa
      421 aggatattca aggaagtatt tagctatctc cattattgga tttccttcaa caactccagc
      481 tgggcagtat gctttcttta tcttagccct aatctcttct ggagagtcat caacagctat
      541 aaaattccct tttgaagaac tcatctttcc ttctccatcc aaacccgtta agacagggtt
      601 gtgaatacaa acaacctttt ttggtaaaag ctcccttgct aacatgtgta tttttctctg
      661 ctccatccct ccaactgcaa catcaacgcc cagataatga ctcgtattaa cctgcattat
      721 tggatagata acttcagcaa cctttggatt ttcatcctct cttgctataa gttccatact
      781 ccttcttgct ctttttaagg tagtttttaa agccaatcta tagacattca gtgtataatc
      841 cttatcaagc tggaaaggac ttccataaag atattttgcc tttaacccca ttgcttcaaa
      901 aacttttttg ttataatctc ctatttttct aatctcatcc aactctcctt tctggtttaa
      961 ataggcgtgt aaatcagcca acaatataat tatatcaaat ccagcatttt gtaaatcaat
     1021 catcttttt
//
