LOCUS       sequence-90376-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of APX W41F inducible by IPTG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Dwyer DJ, Belenky PA, Yang JH, MacDonald IC, Martell JD, Takahashi 
            N, Chan CT, Lobritz MA, Braff D, Schwarz EG, Ye JD, Pati M, 
            Vercruysse M, Ralifo PS, Allison KR, Khalil AS, Ting AY, Walker GC, 
            Collins JJ
  TITLE     Antibiotics induce redox-related physiological alterations as part 
            of their lethality.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 May 20;111(20):E2100-9. doi: 
            10.1073/pnas.1401876111. Epub 2014 May 6.
  PUBMED    24803433
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1401876111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-05-20- 20"; volume: "111"; issue: "20"; pages: "E2100-9"
COMMENT     Sequence Label: pTRC-APX(W41F) dimeric
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggcacacc accaccacca ccacaaggat ccaggaaagt catacccaac cgtgagccca
       61 gattaccaga aggctattga gaaggcaaaa aggaaactga ggggattcat cgcagaaaag
      121 aaatgcgccc cactgatcct gcgactggct ttccacagcg caggaacatt tgactccaag
      181 accaaaacag gcggaccctt tgggactatc aagcaccagg ctgaactggc acatggggcc
      241 aacaatggcc tggacattgc tgtgagactg ctggagccaa tcaaagaaca gttccccatt
      301 gtctcctacg cagacttcta ccagctggcc ggcgtggtcg ctgtggagat taccggcggc
      361 cccgaggtcc ctttccaccc aggaagggag gacaaacctg aaccccctcc agaggggaga
      421 ctgccagatg ctaccaaggg cagcgaccat ctgcgggacg tgttcggaaa agcaatgggg
      481 ctgtctgacc aggatatcgt ggccctgagt ggagggcaca ccattggcgc cgctcataag
      541 gaacgctcag gcttcgaggg accctggaca agcaaccctc tgatcttcga caattcctac
      601 tttactgaac tgctgaccgg cgagaaggat ggactgctgc agctgccctc tgacaaagcc
      661 ctgctgacag atagcgtgtt caggcctctg gtcgaaaagt atgcagccga cgaggatgtg
      721 ttcttcgccg actatgctga ggcacacctg aaactgtctg aactgggatt cgccgaggca
      781 gggaattctt aa
//
