LOCUS       sequence-90379-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  split Venus attached to MCP and PCP respectively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Wu B, Chen J, Singer RH
  TITLE     Background free imaging of single mRNAs in live cells using split 
            fluorescent proteins.
  JOURNAL   Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
  PUBMED    24402470
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-01-9"; volume: "4:3615. doi"; pages: " 
            10.1038/srep03615"
COMMENT     Sequence Label: ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             75..95
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             126..137
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      534..543
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(585..606)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg
       61 cggccgccat gggcccaaaa aagaaaagaa aagttggcta cccctacgac gtgcccgact
      121 acgccatcga aggccgccat atgctagccg ttaaaatggc ttctaacttt actcagttcg
      181 ttctcgtcga caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg
      241 ggatcgctga atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg
      301 ttcgtcagag ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaaggcg
      361 cctggcgttc gtacttaaat atggaactaa ccattccaat tttcgccacg aattccgact
      421 gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg attccctcag
      481 caatcgcagc aaactccggc atctacgcgg attctagacg ggatccaccg gtcgccacca
      541 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      601 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      661 gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgcct tggcccaccc
      721 tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc
      781 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccagga
//
