LOCUS       sequence-90381-e         773 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  split Venus attached to MCP and PCP respectively.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Wu B, Chen J, Singer RH
  TITLE     Background free imaging of single mRNAs in live cells using split 
            fluorescent proteins.
  JOURNAL   Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038/srep03615.
  PUBMED    24402470
REFERENCE   2  (bases 1 to 773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-01-9"; volume: "4:3615. doi"; pages: " 
            10.1038/srep03615"
COMMENT     Sequence Label: ubc-nls-ha-MCP-VenusN-nls-ha-PCP-VenusC
FEATURES             Location/Qualifiers
     source          1..773
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      373..382
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             385..405
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             409..435
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             409..435
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             436..447
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
       61 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
      121 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
      181 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
      241 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
      301 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
      361 cgtcacttgg cggccgccat gggcccaaaa aagaaaagaa aagttggcta cccctacgac
      421 gtgcccgact acgccatcga aggccgccat atgctagccg ttaaaatggc ttctaacttt
      481 actcagttcg ttctcgtcga caatggcgga actggcgacg tgactgtcgc cccaagcaac
      541 ttcgctaacg ggatcgctga atggatcagc tctaactcgc gttcacaggc ttacaaagta
      601 acctgtagcg ttcgtcagag ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg
      661 cctaaaggcg cctggcgttc gtacttaaat atggaactaa ccattccaat tttcgccacg
      721 aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tgg
//
