LOCUS       sequence-90397-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the PLtetO-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL6
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             132..143
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(153..178,179..184,185..201,202..207,
                     208..226))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    183..201
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    208..226
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(302..319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
       61 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
      121 ccatgggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
      181 tatctctatc actgataggg atgtcaatct ctatcactga tagggatact aagggcaagg
      241 tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt
      301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      361 caaaggcggt aatctcgagg ttacattgtc gatctgttca tggtgaacag ctttgaatgc
      421 accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca
      481 tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt tgtttgttag
      541 tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag
      601 ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga
      661 gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt
      721 ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag gaatctgatg
      781 taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta
      841 cgagatccat ttgtctatct agttcaactt ggaaaa
//
