LOCUS       sequence-90399-e         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mCherry under the PLtetO-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL6
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(17..63)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(72..782)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(350..369)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     619..637
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     RBS             790..801
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(811..836,837..842,843..859,860..865,
                     866..884))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    866..884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccgtaacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
       61 ttgatgcctg gttacttgta cagctcgtcc atgccgccgg tggagtggcg gccctcggcg
      121 cgttcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg
      181 ttgacgttgt aggcgccggg cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc
      241 tcagcgtcgt agtggccgcc gtccttcagc ttcagcctct gcttgatctc gcccttcagg
      301 gcgccgtcct cggggtacat ccgctcggag gaggcctccc agcccatggt cttcttctgc
      361 attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta gatgaactcg
      421 ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc
      481 acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg gatgtcggcg
      541 gggtgcttca cgtaggcctt ggagccgtac atgaactgag gggacaggat gtcccaggcg
      601 aagggcaggg ggccaccctt ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg
      661 cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc
      721 accttgaagc gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcacc
      781 atgggtacct ttctcctctt taatgaattc ggtcagtgcg tcctgctgat gtgctcagta
      841 tctctatcac tgatagggat gtcaatctct atcactgata gggatactaa gggcaaggtg
      901 tcaccaccct gccc
//
