LOCUS       sequence-90415-e         744 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  {Ud} is composed of two genes; (1) a UAS-RpL14.dsRNA targeting RNAi 
            to a haploinsufficient gene RpL14 and (2) an RNAi insensitive RpL14 
            rescue.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 744)
  TITLE     underdominant attB transformation DM plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: {Ud}RpL14.Dm
FEATURES             Location/Qualifiers
     source          1..744
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..203
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     polyA_signal    232..366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(256..275)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     310..329
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(638..658)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggacgccgt cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg
       61 agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca
      121 tcatgatgga ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct
      181 ggcacccgca ccgcggcttc gagaccgtga cctacatcgt cgacactagt ggatccagac
      241 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      301 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      361 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      421 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctctagtca
      481 aggcactata catcaaatat ttttccataa ttttcttgta tagcagtgca gctttttcct
      541 ttgtggtgta aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa
      601 aacttagcaa ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag
      661 gagtagaatg ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc
      721 aaaacaggtt ttcctcatta aagg
//
