LOCUS       sequence-90418-e        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the 
            PcpcG2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL4
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtcttaa
       61 tttttttgac ggtaaagcca agctgaaggc tgacaaaatc tatactgtta tatccccctt
      121 tcctggggga cttaggcggt ttaataactg gggtttttgc tagaaatatt ttggcgttta
      181 attatttatt ctctggttta atatttcgag actttgcaga ctttgtattt gaggccagac
      241 tccaatgttg aaactagccg gtaaaaaagc ctctgccctg acctgcaaga aaacgaaggc
      301 aaaaattggt atcctagaaa tggatcggca gtggtatcaa tcagtcaagc gatttttatg
      361 tccaagtttt gaaatttctg ctatcaactt ccaagatatt ttgtttaaca atgttgacgt
      421 tggagaatat cattcgatcc tagttggttg cgggattaaa tatgaatcaa ttgatatttc
      481 agctacttta gatttagtga ccattattaa aaaattgtct gctaagccgc cggccctact
      541 actaattacg gattgtgccg acagtcagat cacggtacaa gctcgcagtt acctgcccca
      601 aattgacggc gtttttgcca aggatcatga tttagcttta ttgttaaaag tcatgaaaat
      661 tattgccaaa caaaaatatt ttaagtaagg tttttctact gggattcgct aatatatggg
      721 caaatttcta attccaatcg aatttgtttt tctggcgatc gccatgacct gttatttatg
      781 gcacagacaa aaccaagaac gccgcaggat tgaaattagc atcaagcaac aaacccaacg
      841 ggaacgattt attaaccaaa ttacccaaca tatccgccaa tctttaaact tggaaacggt
      901 tttaaatacc accgtcgctg aagttaaaac cctgttgcaa gttgatcgag ttctaattta
      961 tcgcatttgg caagatggca cgggcagcgc cattacggaa tcggtgaatg ccaattatcc
     1021 tagtatttta gggcggacct tttccgatga agtttttccc gttgaatacc atcaagccta
     1081 caccaaaggt aaagtacggg ccattaatga cattgaccag gatgacatag agatttgcct
     1141 agctgatttc g
//
