LOCUS       sequence-90422-e        1138 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the 
            PcpcG2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL4
FEATURES             Location/Qualifiers
     source          1..1138
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(49..151)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
       61 agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg
      121 gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaga tatcgacgtc
      181 ctaagctcga ggcaaatggt tatagcgttt taaacagata gtaactgttg ttccctggtc
      241 aactgcactt tttagcaaga tactgccact gtgtaagtca acacatttct tggcaaccat
      301 taaaccgagt cctgttcccg taatatttct gacatttttg ccccgatgaa agggttcaaa
      361 aatttgcttt tggtcctctg gcgaaatgcc aatgccctgg tcggtgactt caaaaataat
      421 ctgttccgaa tctaggctta gggcaatttt aatctgccct cccccgggag agtatttaat
      481 cgcattagat aacagattag ataaaataga ccgcactaac ctttcatcca ccaatgcctt
      541 cgtatcttga gcgctacaaa taaagtcaaa ataatattgg tcactgacac ttaattgaat
      601 ttcttcgata aattgctgga acaataattt caaatctaac caattaggat taaattccaa
      661 tttgcccgct tcggcacggt taatgattaa aatatcatcc aggagctgta ccatattttt
      721 cacggaattt tgaatacggt gtaagttccg gctacgctta tcgggatcaa gccaggccac
      781 ttcagaattt tccagtaatt gggcagcagc taaggccgta ctgaggggag tacgaaattc
      841 atgggaagcc atggagaaaa aacgagtttt taggcggctt aattcttttt cccgttctaa
      901 agctaggcgg acttcctccg tgcgtcggcg ctcagtgaca tccctttgca cagcaatcca
      961 atgggtgtaa aaacctgttt tattggcaac gggcaccaga ctaaattcca cccaaaactc
     1021 actgccatcc ttacgataat taatcacttc aacggtaact gattgccatt gactaatggc
     1081 ttgccgcacc ctatctaatt cagtgcgact agtttttggt ccctgtaaaa ctcggggg
//
