LOCUS       sequence-90424-e        1097 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the 
            PcpcG2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL4
FEATURES             Location/Qualifiers
     source          1..1097
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(1..47)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1002..1013
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctctagact
       61 agtttttccc ttggcacaaa gatttttccg ttaaattggc cagacgatac ccaatgccat
      121 ggaccgtttc aatggcatct gctgataaac cggcactttt taatttttgt cgcaaactcc
      181 gcacatgcac cttaaccgta tcttcctctg ggggactctc caacttccag atactatcga
      241 taatcatgct ccgacttagc acccgacggc cattgcggag tagtaattcc agaatgctgt
      301 attccttgcg ggtcaaagac aaaacctcat tgtcataact aacttcatag gtgcttggat
      361 ccaacctgat tggcccccac tctaaaactg gttggcacgt tgcacaaccc cgacgcaaca
      421 aagctcgcac cctggcaaat aactccccca aatccactgg cttgaccaca taatcatccg
      481 cccccgcatc caagcccgtg attttatcat tgatcgtatc cctggctgtc atcatcaaaa
      541 ttggcattaa ataactgtgc gatcgccatt tttgacagag ggtaatcccg tccaactccg
      601 gcagcatcac atccaaaata acgaggtcat attccagtcg ggaggcatag tcccaggcga
      661 gggaagcgtc ggtggcaata tcaacggtgt aaagctggtc actcaatgct tcagcaaggg
      721 tttccgccag cggcaaatca tcctccacta aaagaattct catagcccat tgtgcttttc
      781 tctatcaacc tcagcttacc tgaaggggtg aacaggtctg ggttaattca tgttgcgaaa
      841 tgtaacagtt ttagtcgcat cagctaactt tccgatttct ttacgatttt ctcccccttt
      901 tcttcaattt tactttgtta ggatcgcatt tttaatgcca acacatacca gttattggct
      961 ggacattaaa caacttttaa gtttaattac taactttatc taaagaggag aaatactaga
     1021 tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg
     1081 atgtcaacgg tcataag
//
