LOCUS       sequence-90426-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli genetic modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pMDIAI
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(58..104)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(114..914)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
ORIGIN
        1 acggcaagtg gacgattatt agggataaca gggtaatgtg taggctggag ctgcttcgaa
       61 gttcctatac tttctagaga ataggaactt cggaatagga acttatgagc tcagccaatc
      121 gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag gaagatcaac
      181 ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg gatcaaccga
      241 gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag ccacctgtcc
      301 gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg taccaacttg
      361 ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc catcaactcg
      421 gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac ctgcccatcg
      481 agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg caatggatca
      541 gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg ggcgctgcgc
      601 tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg tgtaacgggc
      661 gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat gacgagcgtc
      721 cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc atcttcgagg
      781 ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt aaccccaagg
      841 ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac attgcactcc
      901 accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa atagtcggtg
      961 gtgataaact tatcatcccc ttttgcttat ggagctgcac atgaacccat tcaaaggccg
     1021 gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat acggcatcag
     1081 ttac
//
