LOCUS       sequence-90427-e        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E coli genetic modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pMDIAI
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..124
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     oriT            131..240
                     /note="incP origin of transfer"
ORIGIN
        1 attacgaact cgtcctcggt tcccggcgat cctctggaga ttatattacc ctgttatccc
       61 taattccggg gatccgtcga cctgcagttc gaagttccta ttctctagaa agtataggaa
      121 cttcgaagtt cccgccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
      181 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
      241 ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
      301 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
      361 tatgcagcgg aaaatgcagc tcacggtaac tgatgccgta tttgcagtac cagcgtacgg
      421 cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg tgcagctcca
      481 taagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg ccgttgatcg
      541 tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata cgaatggcga
      601 aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg tgtgctgctg
      661 gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg actgatcgag
      721 gccctgcgtg ctgcgctggg tccgggaggg acgctcgtca tgccctcgtg gtcaggtctg
      781 gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg agttgtctct
      841 gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc ctttgcggca
      901 gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc tcactcgcct
      961 gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct cggcgtggga
     1021 cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc ctatggggtg
     1081 ccgagacact gcaccattct
//
