LOCUS       sequence-90431-e        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the 
            PcpcG2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL4
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(553..572)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ccggaaggct atgtgcagga acgcacgatt tcctttaagg atgacggcac gtacaaaacg
       61 cgtgcggaag tgaaatttga aggcgatacc ctggtaaacc gcattgagct gaaaggcatt
      121 gactttaaag aagacggcaa tatcctgggc cataagctgg aatacaattt taacagccac
      181 aatgtttaca tcaccgccga taaacaaaaa aatggcatta aagcgaattt taaaattcgc
      241 cacaacgtgg aggatggcag cgtgcagctg gctgatcact accagcaaaa cactccaatc
      301 ggtgatggtc ctgttctgct gccagacaat cactatctga gcacgcaaag cgttctgtct
      361 aaagatccga acgagaaacg cgatcatatg gttctgctgg agttcgtaac cgcagcgggc
      421 atcacgcatg gtatggatga actgtacaaa tgatgatcac acaggaaacc tactagatga
      481 tgtctcgttt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      541 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      601 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      661 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
      721 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
      781 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
      841 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgcagtg gggcatttta
      901 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
      961 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     1021 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     1081 ttaaatgtg
//
