LOCUS       sequence-90433-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cloning backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pKSI-1
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        44..62
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    75..182
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     91..107
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     92..108
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(190..209)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(191..209)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(254..271)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(254..270)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(263..285)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      412..867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(499..518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     709..730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 agcgcgcaat taccctgtta tccctaccag gcctctgctg gccaattaac cctcactaaa
       61 gggaacaaaa gctgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata
      121 tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc gcggtggagc
      181 tccaattcgc cctatagtga gtcgtattac accatcaaag cgctggctag ggataacagg
      241 gtaatgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
      301 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
      361 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
      421 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      481 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      541 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      601 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
      661 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      721 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
      781 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
      841 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
      901 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
//
