LOCUS       sequence-90434-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CcaS and CcaR constitutively, and sfGFP/TetR under the 
            PcpcG2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ
  TITLE     Characterizing bacterial gene circuit dynamics with optically 
            programmed gene expression signals.
  JOURNAL   Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884.
  PUBMED    24608181
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884."
COMMENT     Sequence Label: pBL4
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..745)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     RBS             752..763
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 atcatctagt aggtttcctg tgtgatcatc atttgtacag ttcatccata ccatgcgtga
       61 tgcccgctgc ggttacgaac tccagcagaa ccatatgatc gcgtttctcg ttcggatctt
      121 tagacagaac gctttgcgtg ctcagatagt gattgtctgg cagcagaaca ggaccatcac
      181 cgattggagt gttttgctgg tagtgatcag ccagctgcac gctgccatcc tccacgttgt
      241 ggcgaatttt aaaattcgct ttaatgccat ttttttgttt atcggcggtg atgtaaacat
      301 tgtggctgtt aaaattgtat tccagcttat ggcccaggat attgccgtct tctttaaagt
      361 caatgccttt cagctcaatg cggtttacca gggtatcgcc ttcaaatttc acttccgcac
      421 gcgttttgta cgtgccgtca tccttaaagg aaatcgtgcg ttcctgcaca tagccttccg
      481 gcatggcgga cttgaagaag tcatgctgct tcatatggtc cggataacga gcaaagcact
      541 gaacaccata agtcagcgtc gttaccagag tcggccaagg taccggcagt ttaccagtag
      601 tacagatgaa cttcagcgtc agtttaccat tagttgcgtc accttcaccc tcgccacgca
      661 cggaaaactt atgaccgttg acatcaccat ccagttccac cagaataggg acgacaccag
      721 tgaacagctc ttcgccttta cgcatctagt atttctcctc tttagataaa gttagtaatt
      781 aaacttaaaa gttgtttaat gtccagccaa taactggtat gtgttggcat taaaaatgcg
      841 atcctaacaa agtaaaattg aagaaaaggg ggagaaaatc gtaaagaaat cggaaagtta
      901 gctgatgcga ctaaaactgt tacatttcgc aacatgaatt aacccagacc tgttcacccc
      961 ttcaggtaag ctgaggttga tagagaaaag cacaatgggc tatgagaatt cttttagtgg
     1021 aggatgattt gccgctggcg gaaacccttg ctgaagcatt gagtgaccag ctttacaccg
     1081 ttgatattgc caccgacgc
//
