LOCUS       sequence-90437-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 
            516 / 580.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, 
            Liu Y, Zhang J, Xu P, Xu T
  TITLE     Rational design of true monomeric and bright photoactivatable 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021.
  PUBMED    22581370
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-05-13- 13"; volume: "9"; 
            issue: "7"; pages: "727-9"
COMMENT     Sequence Label: mEos3.2-C1
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             39..716
                     /codon_start=1
                     /product="improved monomeric variant of green-to-red 
                     photoswitchable fluorescent protein EosFP (Zhang et al., 
                     2012)"
                     /label=mEos3.2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     misc_feature    717..782
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat gagtgcgatt aagccagaca
       61 tgaagatcaa actccgtatg gaaggcaacg taaacgggca ccactttgtg atcgacggag
      121 atggtacagg caagcctttt gagggaaaac agagtatgga tcttgaagtc aaagagggcg
      181 gacctctgcc ttttgccttt gatatcctga ccactgcatt ccattacggc aacagggtat
      241 tcgccaaata tccagacaac atacaagact attttaagca gtcgtttcct aaggggtatt
      301 cgtgggaacg aagcttgact ttcgaagacg ggggcatttg caacgccaga aacgacataa
      361 caatggaagg ggacactttc tataataaag ttcgatttta tggtaccaac tttcccgcca
      421 atggtccagt tatgcagaag aagacgctga aatgggagcc ctccactgag aaaatgtatg
      481 tgcgtgatgg agtgctgacg ggtgatattg agatggcttt gttgcttgaa ggaaatgccc
      541 attaccgatg tgacttcaga actacttaca aagctaagga gaagggtgtc aagttaccag
      601 gcgcccactt tgtggaccac tgcattgaga ttttaagcca tgacaaagat tacaacaagg
      661 ttaagctgta tgagcatgct gttgctcatt ctggattgcc tgacaatgcc agacgatccg
      721 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
      781 ccaccggatc tagataactg atcataatca gccataccac atttgtagag gtt
//
