LOCUS       sequence-9044-ex        3661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Merritt C, Rasoloson D, Ko D, Seydoux G
  TITLE     3' UTRs are the primary regulators of gene expression in the C. 
            elegans germline.
  JOURNAL   Curr Biol. 2008 Oct 14;18(19):1476-82. doi: 
            10.1016/j.cub.2008.08.013. Epub 2008 Sep 25.
  PUBMED    18818082
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2008.08.013"; journalName: "Curr Biol"; date: 
            "2008-10-14- 14"; volume: "18"; issue: "19"; pages: "1476-82"
COMMENT     Sequence Label: pCM5.41
FEATURES             Location/Qualifiers
     source          1..3661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..747)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1770..1865)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1902..1921)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1903..1921)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1926..1942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1926..1942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2044..2063)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2055..2864
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2122..2141)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3011..3599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3500..3519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg attcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtgggat aaactgtaaa atcgtgcaga aaaatacata
      781 ctgtgttgtt cacgtgtagt acaccgtaaa tcgacacagg ttgctgcctt tgtggttcaa
      841 atcctacccg ttgattgctt atccgttaga atattttaat aaatcgtcct tcttctacaa
      901 atgttctttt agtgattttt tcgtatttca gacatatcct tcctcggctc ctcgttctcc
      961 atcttgtgcc atcgaagcag cagcagtatc acaagttgga ggccttctaa ctccaccacc
     1021 aacaagctgt tcggcgtcac ctcaatcgag taatcactcg gcaaagtcat ttcactagaa
     1081 gaattcaccc cttcttataa tttttcatca tattttttca tccattttta tcgtcctcat
     1141 gcagctagat gtacaaataa tacaactcta ctacctgtta tgtttcctag ttgtcatacc
     1201 attgtgatat tctttctttt tggtagatcc acttcctgtc ccatgactgt ccttttttat
     1261 gtctcgacgt tcttttctct tgtgaaatga ccctaccatg atgcctgttt taccaaatca
     1321 ataattttaa accaccacca ccacaacgac acgtgtctct ttcccttttc attaccgtat
     1381 agttcatttc ccccactacg acactttcca tacaatctct gtaccttatt tttctctcat
     1441 ttgtcagact ttcaaaattg agttttctct ttttcccatc atctccccta aaaccacttt
     1501 cccgagaaaa tccgcaacat tgttattgcc atgaccttac ttttccctcc caattctcaa
     1561 tcagcaccac catttcctgc tatcacactt tctccccatt cactgatttc agcctcgtct
     1621 aattcaccta aattttcata attgttctcg attttatctt tttcttctct cattcaaatc
     1681 accgactctg aactttttag tgaacgccga gtctttgatt cccccccccc cccccccaat
     1741 ttagttgaaa catctatata tacgcatacc aactttatta tacaaagttg gcattataaa
     1801 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     1861 tatttggagc tccatggtag cgttaacgcg gccgcgatat cccctatagt gagtcgtatt
     1921 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     1981 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     2041 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     2101 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     2161 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     2221 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     2281 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     2341 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2401 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2461 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2521 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2581 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2641 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2701 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2761 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2821 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     2881 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     2941 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3121 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     3181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
     3421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3661 t
//
