LOCUS       sequence-90441-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-mCherry1-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..811
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 ggataacatg gccatcatta aggagttcat gcgcttcaag gtgcacatgg aggggtccgt
      181 gaacggccac gtgttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcacctggg acatcctgag
      301 ccctcagttc atgtacggct ccaatgccta cgtgaagcac cccgccgaca tccccgacta
      361 ctttaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaaat tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      481 gaagctgcgc ggcaccaact tcccctccga cggccccgtg atgcagaaga agaccatggg
      541 ctgggaggcc ctctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgaggtcaa
      601 gcccagagtg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aaccgcaagc tggacatcac
      721 cagccacaac gaggactaca ccatcgtgga gcagtacgag agagccgagg gccgccactc
      781 caccggcggc atggacgagc tgtacaagta agcggccgcg actctagatc ataatcagcc
      841 ataccacatt tgtagaggtt
//
