LOCUS       sequence-90444-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 558, Emission: 605.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mRuby-N1
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             105..782
                     /codon_start=1
                     /product="monomeric variant of the red fluorescent protein 
                     eqFP611 (Kredel et al., 2009)"
                     /label=mRuby
                     /translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
                     IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
                     MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
                     VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
                     GLGG"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgaac agcctgatca
      121 aagaaaacat gcggatgaag gtggtgctgg aaggcagcgt gaacggccac cagttcaagt
      181 gcaccggcga gggcgagggc aacccctaca tgggcaccca gaccatgcgg atcaaagtga
      241 tcgagggcgg acctctgccc ttcgccttcg acatcctggc cacatccttc atgtacggca
      301 gccggacctt catcaagtac cccaagggca tccccgattt cttcaagcag agcttccccg
      361 agggcttcac ctgggagaga gtgaccagat acgaggacgg cggcgtgatc accgtgatgc
      421 aggacaccag cctggaagat ggctgcctgg tgtaccatgc ccaggtcagg ggcgtgaatt
      481 ttcccagcaa cggcgccgtg atgcagaaga aaaccaaggg ctgggagccc aacaccgaga
      541 tgatgtaccc cgctgacggc ggactgagag gctacaccca catggccctg aaggtggacg
      601 gcggagggca cctgagctgc agcttcgtga ccacctaccg atccaagaaa accgtgggca
      661 acatcaagat gcccggcatc cacgccgtgg accaccggct ggaaaggctg gaagagtccg
      721 acaacgagat gttcgtggtg cagcgggagc acgccgtggc caagttcgcc ggcctgggcg
      781 ga
//
