LOCUS       sequence-90448-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Fiolka R, Shao L, Rego EH, Davidson MW, Gustafsson MG
  TITLE     Time-lapse two-color 3D imaging of live cells with doubled 
            resolution using structured illumination.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5311-5. doi: 
            10.1073/pnas.1119262109. Epub 2012 Mar 19.
  PUBMED    22431626
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1119262109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-04-3- 3"; volume: "109"; issue: "14"; pages: "5311-5"
COMMENT     Sequence Label: tdTomato-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(234..252)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     502..521
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     696..719
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca agggcgagga
      121 ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag ggctccatga acggccacga
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
      241 gaaggtgacc aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcat
      301 gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc cccgattaca agaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gtctggtgac
      421 cgtgacccag gactcctccc tgcaggacgg cacgctgatc tacaaggtga agatgcgcgg
      481 caccaacttc ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      541 caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc gagatccacc aggccctgaa
      601 gctgaaggac ggcggccact acctggtgga gttcaagacc atctacatgg ccaagaagcc
      661 cgtgcaactg cccggctact actacgtgga caccaagctg gacatcacct cccacaacga
      721 ggactacacc atcgtggaac agtacgagcg ctccgagggc cgccaccacc tgttcctg
//
