LOCUS       sequence-90465-e         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 
            515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PA-mCherry1-C1
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein (Subach et al., 2009)"
                     /label=PAmCherry1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHVFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMKF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEALSERMYPEDGALK
                     GEVKPRVKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    741..806
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat taaggagttc atgcgcttca aggtgcacat ggaggggtcc gtgaacggcc
      121 acgtgttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggc ggccccctgc ccttcacctg ggacatcctg agccctcagt
      241 tcatgtacgg ctccaatgcc tacgtgaagc accccgccga catccccgac tactttaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa attcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg tgatgcagaa gaagaccatg ggctgggagg
      481 ccctctccga gcggatgtac cccgaggacg gcgccctgaa gggcgaggtc aagcccagag
      541 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaaccgcaa gctggacatc accagccaca
      661 acgaggacta caccatcgtg gagcagtacg agagagccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
      781 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      841 ccacatttgt agaggtt
//
