LOCUS       sequence-90472-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: C1 Cloning Vector, Excitation: 475, Emission: 505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AcGFP1-C1
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..755
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     misc_feature    756..821
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgccgagc
       61 tgttcaccgg catcgtgccc atcctgatcg agctgaatgg cgatgtgaat ggccacaagt
      121 tcagcgtgag cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      181 tctgcaccac cggcaagctg cctgtgccct ggcccaccct ggtgaccacc ctgagctacg
      241 gcgtgcagtg cttctcacgc taccccgatc acatgaagca gcacgacttc ttcaagagcg
      301 ccatgcctga gggctacatc caggagcgca ccatcttctt cgaggatgac ggcaactaca
      361 agtcgcgcgc cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc gagctgaccg
      421 gcaccgattt caaggaggat ggcaacatcc tgggcaataa gatggagtac aactacaacg
      481 cccacaatgt gtacatcatg accgacaagg ccaagaatgg catcaaggtg aacttcaaga
      541 tccgccacaa catcgaggat ggcagcgtgc agctggccga ccactaccag cagaataccc
      601 ccatcggcga tggccctgtg ctgctgcccg ataaccacta cctgtccacc cagagcgccc
      661 tgtccaagga ccccaacgag aagcgcgatc acatgatcta cttcggcttc gtgaccgccg
      721 ccgccatcac ccacggcatg gatgagctgt acaagtccgg actcagatct cgagctcaag
      781 cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataactga
      841 tcataatcag ccataccaca tttgtagagg tt
//
