LOCUS       sequence-90484-e        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse NRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: NRAS
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(652..674)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(819..837)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        905..1009
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatccactg agtacaaact ggtggtggtt ggagcaggtg
       61 gtgttgggaa aagcgccttg acgatccagc taatccagaa ccactttgtg gatgaatatg
      121 atcccaccat agaggattct taccgaaagc aagtggtgat tgatggtgag acctgcctgc
      181 tggacatact ggacacagct ggacaagagg agtacagtgc catgagagac cagtacatga
      241 ggacaggcga agggttcctc tgtgtatttg ccatcaataa tagcaaatca tttgcagata
      301 ttaacctcta cagggagcaa attaagcgtg tgaaagattc tgatgatgtc cccatggtgc
      361 tggtaggcaa caagtgtgac ttgccaacaa ggacagttga cacaaagcaa gcccacgaac
      421 tggccaagag ttacggaatt ccattcattg agacctcagc caagacccga cagggtgtgg
      481 aggatgcctt ttacacactg gtaagggaga tacgccagta ccgaatgaaa aagctcaaca
      541 gcagtgacga tggcactcaa ggttgtatgg ggctgccctg tgtgctgatg tagcaattca
      601 tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
      661 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      721 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
      781 tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt
      841 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
      901 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      961 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagt
//
