LOCUS       sequence-90489-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 
            516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: tdEos-N1
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     CDS             105..782
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatgagt gcgattaagc
      121 cagacatgaa gatcaacctc cgtatggaag gcaacgtaaa cgggcaccac tttgtgatcg
      181 acggagatgg tacaggcaag ccttttgagg gaaaacagag tatggatctt gaagtcaaag
      241 agggcggacc tctgcctttt gcctttgata tcctgaccac tgcattccat tacggcaaca
      301 gggtattcgc cgaatatcca gaccacatac aagactattt taagcagtcg tttcctaagg
      361 ggtattcgtg ggaacgaagc ttgactttcg aagacggggg catttgcatt gccagaaacg
      421 acataacaat ggaaggggac actttctata ataaagttcg atttcacggt gtaaactttc
      481 ccgccaatgg tccagttatg cagaagaaga cgctgaaatg ggagccctcc actgagaaaa
      541 tgtatgtgcg tgatggagtg ctgactggtg atattcgcat ggctttgttg cttgaaggaa
      601 atgcccatta ccgatgtgac ttcagaacta cttacaaagc taaggagaag ggtgtcaagt
      661 taccaggcta ccactttgtg gaccactgca ttgagatttt aagccatgac aaagattaca
      721 acaaggttaa gctgtatgag catgctgttg ctcattctgg attgcctgac aatgccagac
      781 gatccggtag cggtcacggt actggttcta ctggttctgg ttcttctgag agtgcgatta
      841 agccagacat gaagatcaac ctccgtatgg
//
