LOCUS       sequence-90490-e        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of mouse KRAS2B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Fritsch R, de Krijger I, Fritsch K, George R, Reason B, Kumar MS, 
            Diefenbacher M, Stamp G, Downward J
  TITLE     RAS and RHO families of GTPases directly regulate distinct 
            phosphoinositide 3-kinase isoforms.
  JOURNAL   Cell. 2013 May 23;153(5):1050-63. doi: 10.1016/j.cell.2013.04.031.
  PUBMED    23706742
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.04"; journalName: "Cell"; date: "2013-05-23- 
            23"; volume: "153"; issue: "5"; pages: "1050-63"
COMMENT     Sequence Label: KRAS2B
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(649..671)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(816..834)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        902..1006
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatccactg agtataaact tgtggtggtt ggagctggtg
       61 gcgtaggcaa gagcgccttg acgatacagc taattcagaa tcactttgtg gatgagtacg
      121 accctacgat agaggactcc tacaggaaac aagtagtaat tgatggagaa acctgtctct
      181 tggatattct cgacacagca ggtcaagagg agtacagtgc aatgagggac cagtacatga
      241 gaactgggga gggctttctt tgtgtatttg ccataaataa tactaaatca tttgaagata
      301 ttcaccatta tagagaacaa attaaaagag taaaggactc tgaagatgtg cctatggtcc
      361 tggtagggaa taagtgtgat ttgccttcta gaacagtaga cacgaaacag gctcaggagt
      421 tagcaaggag ttacgggatt ccgttcattg agacctcagc aaagacaaga cagggtgttg
      481 acgatgcctt ctatacatta gtccgagaaa ttcgaaaaca taaagaaaag atgagcaaag
      541 atgggaagaa gaagaagaag aagtcaagga caaggtgtac agttatgtga gaattcatcg
      601 tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      661 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      721 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
      781 cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
      841 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
      901 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      961 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattc
//
