LOCUS       sequence-90493-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya-N1
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..97
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..818
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSFLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMYLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag cttcgaattc
       61 tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc caccatggtg agcaagggcg
      121 aggggcaatc gaagcatggg ctaaaggagg agatgacggt gaagtaccac atggaaggtt
      181 gcgttaatgg tcacaaattt gtcattactg gagagggcat tggaaaccct tttaagggta
      241 agcaaactgc aaatttgtgt gtgatagaag gaggcccgct gccgttctcg gaggacattt
      301 taagtccggg ttttaaatat ggtgaccgga ttttcacaga gtacccacag gatattgtag
      361 attacttcaa gaactcatgt ccagcgggct atacgtggga aaggagcttc ctctttgagg
      421 acggagcggt ctgtcgatgc aacgtggaca taacagtctc tgaaaaggag aactgcatct
      481 atcacaaaag tatcttcaga ggggtgaatt ttcccgccga cggccccgta atgaagaaaa
      541 tgaccactaa ttgggaagct agtaccgaga aaattgtgcc tgttccaaag caagggatat
      601 taaaaggaaa ggtcaaaatg tacctattgc tgaaggatgg cggtcgttat cattgccagt
      661 ttgatacggt atataaagct aaatcagtgc cctccaagat gccagaatgg cattttatac
      721 agcataagct ccttagggag gaccgctctg atgctaaaaa ccagaaatgg caattaacag
      781 aacatgcaat cgcaggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata
      841 atcagccata ccacatttgt agaggtt
//
