LOCUS       sequence-90502-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 530, Emission: 541.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, 
            Davidson MW, Campbell RE
  TITLE     An engineered monomeric Zoanthus sp. yellow fluorescent protein.
  JOURNAL   Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 
            10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3.
  PUBMED    24094838
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2013.08.008"; journalName: "Chem Biol"; date: 
            "2013-10-24- 24"; volume: "20"; issue: "10"; pages: "1296-304"
COMMENT     Sequence Label: mPapaya1-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..814
                     /codon_start=1
                     /product="monomeric yellow fluorescent protein derived from
                     the Zoanthus fluorescent protein zFP538"
                     /label=mPapaya1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEGQSKHGLKEEMTVKYHMEGCVNGHKFVITGEGIGNPFKG
                     KQTANLCVIEGGPLPFSEDILSPGFKYGDRIFTEYPQDIVDYFKNSCPAGYTWERSYLF
                     EDGAVCRCNVDITVSEKENCIYHKSIFRGVNFPADGPVMKKMTTNWEASTEKIVPVPKQ
                     GILKGKVKMCLLLKDGGRYHCQFDTVYKAKSVPSKMPEWHFIQHKLLREDRSDAKNQKW
                     QLTEHAIAGMDELYK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtcgccacc atggtgagca agggcgaggg
      121 gcaatcgaag catggactaa aggaggagat gacggtgaag taccacatgg aaggttgcgt
      181 taatggtcac aaatttgtca ttactggaga gggcattgga aaccctttta agggtaagca
      241 aaccgcaaat ttgtgtgtga tagaaggagg cccgctgccg ttctcggagg acattttaag
      301 tccgggtttt aaatatggtg accggatttt cacagagtac ccacaggata ttgtagatta
      361 cttcaagaac tcatgtccag cgggctatac gtgggaaagg agctacctct ttgaggacgg
      421 agcggtctgt cgatgcaacg tggacataac agtctctgaa aaggagaact gcatctatca
      481 caaaagtatc ttcagagggg tgaattttcc cgccgacggc cccgtaatga agaaaatgac
      541 cactaattgg gaagctagta ccgagaaaat tgtgcctgtt ccaaagcaag ggatattaaa
      601 gggaaaggtc aaaatgtgcc tgttgctgaa ggatggcggt cgttatcatt gccagtttga
      661 tacggtatat aaagctaaat cagtgccctc caagatgcca gaatggcatt ttatacagca
      721 taagctcctt agggaggacc gctctgatgc taaaaaccag aaatggcaat taacagaaca
      781 tgcaatcgca ggcatggacg agctgtacaa gtaagcggcc gcgactctag atcataatca
      841 gccataccac atttgtagag gtt
//
