LOCUS       sequence-90504-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mRaspberry-N1
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    18..98
                     /label=MCS
                     /note="multiple cloning site"
     CDS             106..786
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(239..257)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     507..526
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     701..724
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt
       61 ctgcagtcga cggtaccgcg ggcccgggat ccaccggtgg ctaccatggt gagcaagggc
      121 gaggaggtca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      181 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      241 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      301 tgcatgtacg gctccaaggg ctacgtgaag caccccgccg acatccccga ctacttgaag
      361 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      421 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      481 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      541 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat gaagatgagg
      601 ctgaagctga aggacggcgg ccactacgac gccgaggtca agaccaccta catggccaag
      661 aagcccgtgc agctgcccgg cgcctacaag accgacatca agctggacat cacctcccac
      721 aacgaggact acaccatcgt ggaacagtac gagcgcgccg agggccgcca ctccaccggc
      781 gcctaagcgg ccgcgactct agatcataat cagccatacc acatttgtag aggtt
//
