LOCUS       sequence-90513-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Davidson Lab
  TITLE     Michael Davidson Empty Backbones
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: mPlum-Lifeact-7
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..79
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             101..781
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     502..521
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     696..719
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgccaccat gggcgtggcc gacttgatca agaagttcga
       61 gtccatctcc aaggaggagg gggatccacc ggtggctacc atggtgagca agggcgagga
      121 ggtcatcaag gagttcatgc gcttcaagga gcacatggag ggctccgtga acggccacga
      181 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaggct
      241 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagatcat
      301 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
      361 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
      421 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga aggtgcgcgg
      481 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
      541 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatgaaga tgaggctgag
      601 gctgaaggac ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc
      661 cgtgcagctg cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga
      721 ggactacacc atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta
      781 agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
//
